Validation of the HAPPI Kids Continuous Age-Specific Pediatric Reference Intervals

Validation of the HAPPI Kids Continuous Age-Specific Pediatric Reference Intervals

Introduction: In order to facilitate the best possible patient care, reference intervals (gathering) was adopted by the laboratory shall correspond to population demographics and, where applicable, the principles of analysis and / or instruments of analysis used. While the guidelines of the Clinical and Laboratory Standards Institute (CLSI) recommended the validation process for gathering discrete, no current recommendations for the validation process for the continuous gathering. This study aimed to validate the recently published, HAPPI Child-togethers continuously, in the routine laboratory.


Methods: At first, the differences in test results between the main research laboratory that contributes to the development of previous gatherings and routine laboratory was assessed using specimens from 77 children were tested in both the laboratory using the Siemens ADVIA 1800 or Centaur / XP / XPT. Then, the validation of gatherings Children HAPPI done using a sample of 279 children tested in the same type of analyzer in routine laboratory. The gathering issued previously been validated if more than 90% of the results in the laboratory routine in RIs.
Results: There was minimal evidence of clinically significant differences in test results between the primary and routine laboratory. The get-together continuously validated after the initial analysis for 16 of the 18 biochemical analytes tested, and after a secondary analysis of two analytes remaining.


Conclusion: This study validates the gathering HAPPI Children in routine laboratory, laboratory accreditation meets the requirements for the evaluation, implementation, and the source of RIs. In addition, this study presents a modification of the current CLSI method for validation of the continuous gathering routine laboratory that will benefit in general.

 Validation of the HAPPI Kids Continuous Age-Specific Pediatric Reference Intervals
Validation of the HAPPI Kids Continuous Age-Specific Pediatric Reference Intervals

[Association of Urinary Phenol Concentration and Blood Biochemistry Index in Coke Oven Workers]

Objective: To explore the relationship phenol concentration of urine and blood biochemical indices in coke oven workers. Methods: From April to May 2019, we studied 771 employs coke oven workers from the coke plant in Taiyuan city, and categorized into groups of benzene-exposed (n = 402) and control group (n = 369) by exposure to benzene at work and urophenol concentration.

All subjects were interviewed face-to-face using a questionnaire including name, age (years), sex, smoking and drinking habits, history of vocational personal, long (years), and labor protection, etc. work post-shift urine sample is detected using gas chromatography-hydrogen detector flame ionization. Fasting venous blood was taken in the morning and centrifuged, the serum separated using bloodResults detected following items automatically: a male-dominated subjects (n = 719, 93.3%), with the average age (42.3 ± 8.2) years and the length of the average work (20.6 ± 8.2) years.

Amino acid-kit ?(for biochemistry)

K007-1KT 1 unit
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Amino acid-kit ?(for biochemistry)

K007-5KT 1 unit
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D-Maltose Monohydrate for bacteriology and biochemistry

59338 100 Gms
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Acetobromo-α-D-galactose

GC2695 5g
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Acetobromo-α-D-galactose

GC2695-10 10
EUR 270.3

Acetobromo-α-D-galactose

GC2695-25 25
EUR 419.5

Acetobromo-α-D-galactose

GC2695-5 5
EUR 195.7

Acetobromo-alpha-D-glucose

GC7520 10g
EUR 217.3

Acetobromo-alpha-D-glucose

GC7520-10 10
EUR 51.4

Acetobromo-alpha-D-glucose

GC7520-100 100
EUR 237.2

Acetobromo-alpha-D-glucose

GC7520-100G 100 g
EUR 322.8

Acetobromo-alpha-D-glucose

GC7520-10G 10 g
EUR 98.4

Acetobromo-alpha-D-glucose

GC7520-25 25
EUR 95

Acetobromo-alpha-D-glucose

GC7520-25G 25 g
EUR 151.2

Acetobromo-alpha-D-glucose

GC7520-50 50
EUR 150.4

Acetobromo-alpha-D-glucose

GC7520-50G 50 g
EUR 217.2

Acetobromo-Alpha-D-Galactose

abx188579-1kg 1 kg
EUR 2865.6

Acetobromo-alpha-d-glucuronicacidmethylester

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Acetobromo-a-D-glucuronic acid methyl ester

GC3316 1g
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Acetobromo-a-D-glucuronic acid methyl ester

GC3316-1 1
EUR 122.2

Acetobromo-a-D-glucuronic acid methyl ester

GC3316-1G 1 g
EUR 183.6

Bromo 2,3,4,6-Tetra-O-acetyl-a-D-galactopyranoside (Acetobromo-a-D-Galactose, Acetobromogalactose)

MBS6075095-10g 10(g
EUR 420

Bromo 2,3,4,6-Tetra-O-acetyl-a-D-galactopyranoside (Acetobromo-a-D-Galactose, Acetobromogalactose)

MBS6075095-5x10g 5x10g
EUR 1730

Bromo 2,3,4,6-Tetra-O-acetyl-a-D-glucopyranoside (Acetobromo-a-D-glucose, Acetobromoglucose)

MBS6084904-10g 10(g
EUR 460

Bromo 2,3,4,6-Tetra-O-acetyl-a-D-glucopyranoside (Acetobromo-a-D-glucose, Acetobromoglucose)

MBS6084904-5x10g 5x10g
EUR 1925

Loperamide Acetobromo-D-glucuronic Acid Methyl Ester

L597495 5mg
EUR 1200

Acetobromo-alpha-D-glucuronic Acid-13C6 Methyl Ester

A162002 75mg
EUR 15000

Acetobromo-α-D-glucuronic Acid Methyl Ester (contains 2% CaCO3)

A162000 10g
EUR 1127
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Phenolphthalein Acetobromo-beta-D-glucuronic Acid Methyl Ester

P318016 1g
EUR 800
Description: 60665-99-8

Bromo 2,3,4-Tri-O-acetyl-a-D-xylopyranoside (Acetobromo-a-D-xylose)

MBS6044372-250mg 250(mg
EUR 505

Bromo 2,3,4-Tri-O-acetyl-a-D-xylopyranoside (Acetobromo-a-D-xylose)

MBS6044372-5x250mg 5x250mg
EUR 2125

Acetobromo-a-D-glucuronic Acid, Methyl Ester (Bromo-2,3,4-tri-O-acetyl-a-d-glucopyranuronic Acid Methyl Ester)

MBS6039995-2g 2(g
EUR 485

Acetobromo-a-D-glucuronic Acid, Methyl Ester (Bromo-2,3,4-tri-O-acetyl-a-d-glucopyranuronic Acid Methyl Ester)

MBS6039995-5x2g 5x2g
EUR 2025

Acetobromogalactose

573034 10.0g
EUR 350

Acetobromofucose

GC7417 250mg
EUR 1374.1

Acetobromofucose

GC7417-1 1
EUR 913.6

Acetobromofucose

GC7417-2 2
EUR 1500.7

Acetobromofucose

GC7417-250 250
EUR 382.3

Acetobromofucose

GC7417-500 500
EUR 577.8

Bromo Heptaacetyl-D-lactoside, Stabilized with 4%Calcium Carbonate (Acetobromo-a-D-lactoside, Bromo 4-O-(2,3,4,6-tetra-O-acetyl- b-D-galactopyranosyl) -2,3,6-tri-O-acetyl-a -D-glucopyranoside)

MBS6086900-2g 2(g
EUR 455

Bromo Heptaacetyl-D-lactoside, Stabilized with 4%Calcium Carbonate (Acetobromo-a-D-lactoside, Bromo 4-O-(2,3,4,6-tetra-O-acetyl- b-D-galactopyranosyl) -2,3,6-tri-O-acetyl-a -D-glucopyranoside)

MBS6086900-5x2g 5x2g
EUR 1890

Biochemical Test Kit for Shigella

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EUR 49.85
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HiMotility™ Biochemical Kit for V. chol

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EUR 68.78
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EUR 98.33
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KBM004-5KT 1 unit
EUR 40.38
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EUR 94.92
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Biochemical Test Kit for Vibrio parahaem

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EUR 50
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HiMotility™ Biochemical Kit for V. parah

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EUR 68.78
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EUR 98.33
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EUR 40.38
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EUR 50.53
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EUR 68.83
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HiMotility™ Biochemical Kit for Shigella

KBM006-20KT 1 unit
EUR 98.33
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EUR 40.38
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Bromo 2,4,6-Tri-O-acetyl-3-O-(2,3,4,6-tetra-O-acetyl -b-D-glucopyranosyl)-a-D-glucopyranoside (Acetobromolaminaribiose)

MBS6051600-5mg 5(mg
EUR 525

Bromo 2,4,6-Tri-O-acetyl-3-O-(2,3,4,6-tetra-O-acetyl -b-D-glucopyranosyl)-a-D-glucopyranoside (Acetobromolaminaribiose)

MBS6051600-5x5mg 5x5(mg
EUR 2210

Acetone extrapure, 99%

66951 500 ml
EUR 1.95
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Biochemical Test Kit - II

65896 1 Kit
EUR 18.82
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Biochemical Test Kit - I

92875 1 Kit
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Acetoxime (Acetone Oxime) extrapure, 98%

89561 25 Gms
EUR 9.45
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Acetophenone extrapure, 99%

15873 500 ml
EUR 4.72
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Acetophenone extrapure AR, 99.5%

22506 500 ml
EUR 5.82
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HiIMViC™ Biochemical Test Kit

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HiIMViC™ Biochemical Test Kit

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Description: HiIMViC™ Biochemical Test Kit

HiIMViC™ Biochemical Test Kit

KB001-5KT 1 unit
EUR 30
Description: HiIMViC™ Biochemical Test Kit

Acetonitrile (ACN) extrapure, 99%

49967 500 ml
EUR 4.58
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Acetonitrile (ACN) extrapure AR, 99.5%

84578 500 ml
EUR 4.79
Description: Part A

HiAssorted™ Biochemical Test Kit

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HiAssorted™ Biochemical Test Kit

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HiAssorted™ Biochemical Test Kit

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Methyl Acetoacetate extrapure AR, 99%

21392 500 ml
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HiDtect Biochemical Esculin Identificati

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HiDtect Biochemical Esculin Identificati

DT014-50DS 1 unit
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Bis(Acetonitrile)Dichloropalladium (II) extrapure, 99%, ~41% Pd

37025 500 Mg
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Cesium Sulphate extrapure for molecular biology, 99.9%

57455 10 Gms
EUR 7.5
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Griess Reagent for Nitrite extrapure

35657 100 ml
EUR 26.18
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Cesium Chloride extrapure AR for molecular biology, 99.9%

22966 25 Gms
EUR 14.63
Description: Part B

Acetonitrile (ACN) extrapure AR, ACS, ExiPlus, Multi-Compendial, 99.5%

82745 500 ml
EUR 5.61
Description: Part A

D-(+)-Fucose extrapure

76007 500 Mg
EUR 54
Description: Part B

D-(+)-Xylose extrapure

84974 100 Gms
EUR 3.15
Description: Part B

D-(-)-Salicin extrapure

10673 5 Gms
EUR 13.5
Description: Part B

D-Lyxose extrapure

35355 1 Gms
EUR 14.18
Description: Part B

D-Ribose extrapure

48259 25 Gms
EUR 4.13
Description: Part B

D-Altrose extrapure

61973 25 Mg
EUR 110.33
Description: Part B

Triton X-100 extrapure for scintillation

30190 500 ml
EUR 4.52
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D-Fructose extrapure

42868 500 Gms
EUR 3.76
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Total cholesterol assay kit (Microplate Reader and Biochemical Analyzer)

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Total cholesterol assay kit (Microplate Reader and Biochemical Analyzer)

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Total cholesterol assay kit (Microplate Reader and Biochemical Analyzer)

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Total cholesterol assay kit (Microplate Reader and Biochemical Analyzer)

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Total cholesterol assay kit (Microplate Reader and Biochemical Analyzer)

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D-Arabinose extrapure

23848 5 Gms
EUR 2.63
Description: Part B

D-Galactose extrapure

88176 25 Gms
EUR 3.75
Description: Part B

D-Biotin extrapure, 99%

18888 1 Gms
EUR 4.88
Description: Part B

D-Serine extrapure, 99%

53457 5 Gms
EUR 2.05
Description: Part A

D-Cellobiose extrapure

85294 5 Gms
EUR 8.25
Description: Part B

D(+)Arabitol (D-Arabinitol) extrapure 99%

74926 1 Gms
EUR 5.48
Description: Part B

D-Leucine extrapure, 99%

61304 5 Gms
EUR 14.18
Description: Part B

D-Mannitol extrapure, 98.5%

24248 500 Gms
EUR 6.43
Description: Part A

D-Prolinol extrapure, 98%

57732 1 ml
EUR 15.38
Description: Part B

D-Tyrosine extrapure, 98%

70600 5 Gms
EUR 13.68
Description: Part A

D-Glutamine extrapure, 99%

17632 1 Gms
EUR 22.5
Description: Part B

D-Histidine extrapure, 99%

38885 5 Gms
EUR 20.73
Description: Part A

D-Amygdalin extrapure, 97%

85441 1 Gms
EUR 10.8
Description: Part B

Ethyl Acetate extrapure AR, 99.5%

66183 500 ml
EUR 2.33
Description: Part A

Butyl Acetate extrapure AR, 99.5%

80870 500 ml
EUR 3.08
Description: Part A

D-Threoninol extrapure, 98%

24815 500 Mg
EUR 45.98
Description: Part B

D-Isoleucine extrapure, 98%

33401 250 Mg
EUR 67.5
Description: Part B

D-Methionine extrapure, 99%

54322 5 Gms
EUR 9.15
Description: Part B

D-Mannose extrapure AR, 99%

13735 25 Gms
EUR 4.5
Description: Part B

D-Valine extrapure CHR, 99%

21562 5 Gms
EUR 6.45
Description: Part B

Z-D-Lysine extrapure, 99%

39218 1 Gms
EUR 71.18
Description: Part B

ß-D-Allose extrapure, 98%

46287 100 Mg
EUR 58.05
Description: Part B

Z-D-Serine extrapure, 99%

63273 500 Mg
EUR 16.35
Description: Part B

Z-D-Valine extrapure, 99%

74556 1 Gms
EUR 9.9
Description: Part B

D-Prolinamide extrapure, 98%

90557 5 Gms
EUR 35.63
Description: Part B

Barium Acetate extrapure, 98%

10013 500 Gms
EUR 6.84
Description: Part A

Acetanilide extrapure, 99%

81658 500 Gms
EUR 8.55
Description: Part A

Z-D-Alanine extrapure, 99%

15701 1 Gms
EUR 4.5
Description: Part B

Z-D-Proline extrapure, 99%

24037 500 Mg
EUR 6.98
Description: Part B

Z-D-Leucine extrapure, 99%

46817 1 Gms
EUR 8.18
Description: Part B

D-Alanine extrapure CHR, 99%

64422 5 Gms
EUR 3.15
Description: Part B

D-Mannitol extrapure AR, 99%

79887 250 Gms
EUR 3.56
Description: Part A

D-Proline extrapure CHR, 99%

95365 5 Gms
EUR 10.26
Description: Part A

D-(-)-Tartaric Acid extrapure, 99%

28682 100 Gms
EUR 4.05
Description: Part B

BOC-D-Valine extrapure, 99%

35049 25 Gms
EUR 28.5
Description: Part B

Z-D-Tyrosine extrapure, 99%

59268 1 Gms
EUR 19.35
Description: Part B

BOC-D-Serine extrapure, 98%

91810 10 Gms
EUR 101.63
Description: Part B

Z-D-Arginine extrapure, 99%

94475 1 Gms
EUR 45.6
Description: Part B

D-(+)-Glyceraldehyde extrapure, 85%

14210 1 Gms
EUR 112.5
Description: Part B

FMOC-D-Valine extrapure, 99%

15917 1 Gms
EUR 10.2
Description: Part B

BOC-D-Leucine extrapure, 99%

22237 10 Gms
EUR 28.5
Description: Part B

Z-D-Norvaline extrapure, 99%

34201 1 Gms
EUR 20.93
Description: Part B

Z-D-Threonine extrapure, 99%

52287 1 Gms
EUR 9.75
Description: Part B

FMOC-D-Serine extrapure, 99%

53534 500 Mg
EUR 17.48
Description: Part B

D-Cysteine (base) extrapure, 99%

73383 1 Gms
EUR 22.95
Description: Part B

Z-D-Glutamine extrapure, 99%

83309 1 Gms
EUR 9.23
Description: Part B

BOC-D-Alanine extrapure, 99%

86204 10 Gms
EUR 26.78
Description: Part B

BOC-D-Proline extrapure, 99%

93481 10 Gms
EUR 26.78
Description: Part B

2-Acetyl Pyridine extrapure, 99%

10197 25 ml
EUR 13.88
Description: Part B

Acetylacetone extrapure AR, 99.5%

24212 250 ml
EUR 4.11
Description: Part A

Thallium (I) Acetate extrapure, 99.9%

36610 5 Gms
EUR 22.28
Description: Part B

Ammonium Acetate extrapure AR, 98%

44683 500 Gms
EUR 2.12
Description: Part A

Isobutyl Acetate extrapure, 98%

52497 500 ml
EUR 3.76
Description: Part A

Acetic Acid Glacial extrapure AR, 99.9%

85801 500 ml
EUR 1.78
Description: Part A

Acetic Acid Glacial extrapure, 99.5%

90868 500 ml
EUR 1.64
Description: Part A

Acetylacetone extrapure, 99%

96169 500 ml
EUR 6.16
Description: Part A

FMOC-D-Leucine extrapure, 99%

16515 1 Gms
EUR 4.73
Description: Part B

Actinomycin D (AMD) extrapure, 98%

28269 2 Mg
EUR 42.23
Description: Part B

Z-D-Tryptophan extrapure, 99%

39868 1 Gms
EUR 16.5
Description: Part B

FMOC-D-Proline extrapure, 99%

53412 5 Gms
EUR 32.48
Description: Part B

Z-D-Norleucine extrapure, 99%

66829 500 Mg
EUR 34.58
Description: Part B

FMOC-D-Alanine extrapure, 99%

77535 1 Gms
EUR 4.8
Description: Part B

Z-D-Methionine extrapure, 99%

91642 1 Gms
EUR 11.25
Description: Part B

Z-D-Asparagine extrapure, 99%

92873 1 Gms
EUR 8.18
Description: Part B

Galantamine Hydrobromide extrapure, 98%

83055 250 Mg
EUR 67.5
Description: Part B

a-Naphthyl Acetate extrapure AR, 99%

43036 5 Gms
EUR 7.5
Description: Part B

Potassium Acetate extrapure AR, 99%

79156 500 Gms
EUR 4.65
Description: Part A

ß-Naphthyl Acetate extrapure AR, 99%

79388 5 Gms
EUR 7.5
Description: Part B

D-Sorbitol Powder extrapure, 98%

14281 500 Gms
EUR 3.63
Description: Part A

BOC-D-Aspargine extrapure, 99%

19362 10 Gms
EUR 35.33
Description: Part B

D-Tryptophan (base) extrapure, 99%

26579 10 Gms
EUR 24.08
Description: Part A

BOC-D-Glutamine extrapure, 99%

45737 10 Gms
EUR 48.75
Description: Part B

FMOC-D-Tyrosine extrapure, 99%

54034 1 Gms
EUR 7.8
Description: Part B

FMOC-D-Arginine extrapure, 99%

66725 1 Gms
EUR 16.35
Description: Part B

BOC-D-Threonine extrapure, 98%

73138 10 Gms
EUR 35.33
Description: Part B

2-Deoxy-D-Ribose extrapure

84384 1 Gms
EUR 3.75
Description: Part B

D-Glucuronic Acid extrapure, 98%

88229 1 Gms
EUR 7.13
Description: Part B

ß-D-Gentiobiose extrapure, 95%

95677 100 Mg
EUR 21
Description: Part B

D-Leucyl-Glycine extrapure, 98%

37520 100 Mg
EUR 18.9
Description: Part B

FMOC-D-Threonine extrapure, 98%

56859 1 Gms
EUR 26.25
Description: Part B

FMOC-D-Glutamine extrapure, 99%

59874 500 Mg
EUR 4.73
Description: Part B

BOC-D-Tryptophan extrapure, 99%

61017 10 Gms
EUR 30
Description: Part B

FMOC-D-Norvaline extrapure, 99%

73648 1 Gms
EUR 28.58
Description: Part B

D-Homophenylalanine extrapure, 97%

94194 500 Mg
EUR 17.85
Description: Part B

Low-density lipoprotein cholesterol(LDL) assay kit (Microplate Reader and Biochemical Analyzer)

MBS2540572-5x96Test 5x96Test
EUR 1145

Compared with the control group, the benzene-exposed group were significantly different in age, long years, sex work, smoking and drinking habits (P <0.05). Median (interquartile interval) urinary phenol concentration was 6.00 (0.00 to 33.00) ug / ml in benzene-exposed group, which was significantly higher than in the control group (P <0.05). analysis of covariance showed that fasting blood glucose, total cholesterol and high density on benzene-exposed group was significantly reduced compared with the control group, but serum creatinine, serum uric acid and triglyceride levels were significantly increased (P <0.05).

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