BACKGROUND: SIGMA metrics provide an objective and quantitative methodology for an assessment of the analytical quality of the clinical laboratory. This study investigated the test performance of validated systems and unsuitable systems based on Sigma metrics and explored the main parameters affecting the system performance.
Methods: Sigma metrics have been evaluated by six biochemistry tests based on validated and not validated systems of Beckman and Mindray by crossing reagents and analyzers. The inaccuracy and biases were evaluated for all tests based on sampling programs organized by the National Clinical Laboratory Center. Total error allowance obtained from the Chinese Health Laboratory Center Standard (WS / T403-2012).
Results: The inaccuracy of all systems meets quality specifications, with the exception of TP (2.19%) detected by an unaulted MinDray system, and the four tests measured by unaulted systems can not Not fulfill the criterion, including lactate Dehydogenase (LDH), total proteins (TP), triglycerides (Tg) and glucose (GLU). Higher biases have been detected in six tests at different levels among uncommitted and validated systems. Little or unacceptable systems for TP test with SIGMA metrics less than 3 unless otherwise validated Mindray. SIGMA metrics for other tests with four systems were greater than 3, with the exception of the LDH evaluated on non-validated MinDray systems.
Conclusion: Not validated systems can introduce performance uncertainty compared to validated systems based on the evaluation of SIGMA metrics and a lower bias has been provided by validated systems. The performance of unaulted systems should be thoroughly assessed in the clinical laboratory before being adopted for routine use.
Reference values for hematology, plasma biochemistry, bone marrow cytology and bone histology of nod.cg-prkdcscid il2rgtm1wjl / szj immunodeficient
Very immunodefic NSG mice are commonly used as models in preclinical studies for the patient’s derived claw. However, despite the frequency of their use, the reference values for their clinical pathology markers have not been determined. In accordance with the American company recommendations of the veterinary clinical pathology (ASVCP), we have created Novo’s reference values for hematological and biochemical variables and evaluated the cytology and histology of bone marrow bones in about 40 mice. Men and women of NSG, aged 9 weeks old. Hematologic analyzes were performed using 2 separate analyzers (Idexx Procuste DX, SysMex XT-2000IV) and biochemical values were measured using a VetScan2 scil.
The primary hematological characteristic seen in NSG mice was a very low number of white blood cells (WBC) (below 1.6 109 / L). The levels of lymphocytes and monocytes were respectively exceeded and underestimated by analyzers, compared to the manual accounts, probably due to poor identification of the very low concentrations of these types of cells by the analyzers. This analytic bias highlights the need for confirmed microscopic observation of the blood smear of these mice for the differential identification of the WBC. The results of all other haematology and biochemistry variables were similar to those previously reported in registered mice, with the exception of MPV and unexpected glucose concentration (11.5 to 19.0 mmol / l), possibly possibly Due to the non-maximum status of animals.
The number of differential bone marrow cells and myeloid: the erythroid ratio (1.76 median 1,76) were also established. Megaaryocytes and the number of adipocytes differ significantly between femoral diaphysis and metaphysics and between the genders. These results are a reliable basic data resource for hematological variables for researchers monitor graft rejection studies in NSG mice.
Sigma metrics application for validated and non-validated detecting systems performance assessment
Adiponectin certifies liver injuries in sepsis rats through a path AmpK / MTOR
Objective: to investigate the influences of adiponectin (APN) on liver injury in sepsy rats and explore if it exerts a therapeutic effect through the Kinase protein target (AMPK) / Mammalian activated by adenosine (ampk) / mammalian of the route of rapamycin (MOR).
Materials and methods: A rat sevencine model has been set up through the Cecal Ligation and Puncture group (CLP Group) and APN Processing Group (APN Group) and Control Group have also been defined. Changes made to hepatic function indicators, Alanine aminotransferase (ALT) and aminotransferase (AST) serum (AST), were determined by automatic biochemistry analyzer and tumor-α necrosis factor levels (TNF- α), interleukin (IL) – 1 and IL-6 were measured via an immunosorbent dosage linked to an enzyme (ELISA). Hematoxylin-eosin (HE) Coloring has been used to detect lesions of hepatic tissue and apoptosis and hepatocyte necrosis after the APN intervention were evaluated using in situ color. fluorescence. In addition, the expression of the APN arna in the hepatic tissues has been detected by a quantitative reaction of the reverse-polymerase transcription chain (QRT-PCR) and the expression levels of MTOR and mTor proteins Phosphorylated in hepatic tissue samples were determined using Western blot.
Results: In terms of evolution of the hepatic function indicators, the Alt and TEN concentrations have been substantially raised in the CLP group and compared to those of the control group, the concentrations of the two indicators have decreased considerably in the APN group , showing statistically significant differences (p <0.05). The CLP and APN group obviously had higher inflammatory factor levels than the control group, but their levels in the APN group were lower than those of the CLP group (P <0.05). It has been found through the staining of the sepsy rats in the CLP group had a massive inflammatory infiltration and that the inflammatory cells have been remarkably diminished in the APN group after APN treatment. According to the results of in situ fluorescence coloring, the CLP group exhibited a significant increase in cell apoptosis level and the APN group had substantially reduced apoptotic cells (p <0.05).
Description: Ureases (EC 3.5.1.5), functionally, belong to the superfamily of amidohydrolases and phosphotriesterases. It is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows: (NH2)2CO + H2O → CO2 + 2NH3.
The results of determining the APN expression revealed that the CLP group had a reduced level of APN and the APN level in the APN group was significantly higher than that of the control group. Based on the results of Western blotting, the level of phosphorylated ampk has been remarkably high and that of phosphorylated mTor has been lowered in the CLP group with respect to those of the control group, while compared to the CLP group, the APN Group showed a considerable increase in phosphorylated ampk level and separate decline in the level of phosphorylated mTor.
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