Crohn’s disease (CD) is characterized by chronic intestinal inflammation and the various factors involved in its pathogenesis including oxidative stress. CD oxidative stress may harm antioxidant nutrients, such as selenium. The purpose of this study was to assess the status of selenium and its relationship with oxidative stress markers in patients with CD compared with controls.The study included 47 patients with CD (20 with active disease and 27 in remission) and 25 healthy people. Blood samples were collected for analysis of plasma and erythrocyte selenium concentration using inductively coupled plasma optical emission spectrometry (ICP-OES).
Selenoprotein P (SEPP) was evaluated with bread enzyme-linked immunosorbent assay, erythrocyte glutathione peroxidase (GPx1) activity was assessed using an automatic biochemical analyzer, and the concentration of thiobarbituric acid reactive substances (TBARS) measured. comparative analysis performed using one-way analysis of variance and post hoc Tukey test. For correlation, Pearson coefficient test used. The deciding factor for CD and lipid peroxidation characterized by ratio.Plasma opportunities and erythrocyte selenium levels and concentration Sepp lower in CD patients compared to the healthy group. GPx1 activity and TBARS concentrations were significantly higher in CD group.
In univariate analysis, plasma and erythrocyte selenium and TBARS associated with CD.Patients with CD have an impaired selenium status, which is associated with increased oxidative stress was observed in these patients.
Here, portable and accurate phosphate sensor using Fabry-Perot gradient array (FPA) was proposed. It can form a concentration gradient in both directions (absorbance) in FPA gradient distribution, simplifies complex operations to obtain a standard curve and save time. FPA gradient can effectively filter out disturbances (bubbles, light intensity and salinity) while increasing the absorbance, achieving highly accurate detection and stable.
In addition, the smartphone simplifies data processing and more portable sensor brand. In this work, fault detection standard solution (100 M, 50 pM and 30 M) was 0.39%, 1.48% and 1.84%, respectively. And it also has been demonstrated with an error of 2.46% (sample 1, sea water), 2.08% (sample 2, the water of the lake) and 1.83% (3 samples, waste) for the detection of natural samples, more accurate than traditional analyzer.
Relationship between selenium status and biomarkers of oxidative stress in Crohn disease.
Multivariate predictive model to estimate the concentration of ionized calcium serum biochemical results in dogs: an external validation.
Predicted ionized calcium (Pica) can be calculated from routine biochemical variables using a prediction model was recently developed in dogs. However, it has not been evaluated by the measured variable of some laboratories.We aims to (a) externally validate Pica in dogs in which the biochemical results obtained from different analysis, and (b) compare the diagnostic performance pica and the amount of calcium (tCa) .A cross -sectional multicenter study in 138 dogs from three different hospitals performed.
Description: For quantitative determination of urease activity and evaluation/screen for urease inhibitors. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.003 U/L urease activity can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD670nm. Samples: Biological, environment etc. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.003 U/L.
Description: EX 527 (SEN0014196) is a novel, potential, and specific small-molecule inhibitor of SIRT1 catalytic activity to examine the role of SIRT1 in p53 acetylation and cell survival after DNA damage
Description: EX 527 (SEN0014196) is a novel, potential, and specific small-molecule inhibitor of SIRT1 catalytic activity to examine the role of SIRT1 in p53 acetylation and cell survival after DNA damage
Description: EX 527 (SEN0014196) is a novel, potential, and specific small-molecule inhibitor of SIRT1 catalytic activity to examine the role of SIRT1 in p53 acetylation and cell survival after DNA damage
Description: Quantitative sandwich ELISA for measuring Mouse Ureaplasma urealyticum antibody (UU-Ab) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse Ureaplasma urealyticum antibody (UU-Ab)
Description: Quantitative sandwich ELISA for measuring Mouse Ureaplasma urealyticum antibody (UU-Ab) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
ELISA kit for Mouse Ureaplasma urealyticum antibody (UU-Ab)
Description: Quantitative sandwich ELISA for measuring Mouse Ureaplasma urealyticum antibody (UU-Ab) in samples from cell culture supernatants, serum, whole blood, plasma and other biological fluids.
Description: Enzyme-linked immunosorbent assay kit for quantification of Human Urea transporter 1 in samples from serum, plasma, tissue homogenates and other biological fluids.
Description: The substance EX-527 is a sirt1 inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is light yellow solid which is soluble in 18 mg/ml DMSO or 10 mg/ml Ethanol.
Description: The substance EX-527 is a sirt1 inhibitor. It is synthetically produced and has a purity of >98%. The pure substance is light yellow solid which is soluble in 18 mg/ml DMSO or 10 mg/ml Ethanol.
Sensitivity (Sen), specificity (Spe), positive (PPV) and negative predictive values (NPV), and clumsiness diagnostic pica and tCa calculated using logistic regression to ionized hypercalcemia and hypocalcemia. fluctuations in hospital diagnostic performance also assessed.For ionized hypercalcemia, which Sen (81.8%), Spe (96.1%), PPV (69.2%), NPV (97.7%), and diagnostic irregularities ( 5.1%) of Pica does not significantly different between hospitals or from those of tCa.
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